Facile Preparation of Organo-Modified ZnO/Attapulgite Nanocomposites Loaded with Monoammonium Glycyrrhizinate via Mechanical Milling and Their Synergistic Antibacterial Effect

In this study, monoammonium glycyrrhizinate (MAG) was introduced into cetyltrimethyl ammonium bromide (CTAB)-modified ZnO/attapulgite (APT) via a mechanical process to form performance-enhanced antibacterial nanocomposites (MAG/C–ZnO/APT). The APT supported ZnO nanocomposite (ZnO/APT) prepared by conventional precipitation method, and 20–50 nm of globular nanoparticles were uniformly decorated on nanorods. FTIR zeta potential analyses demonstrated that modification CTAB facilitated the loading MAG ZnO/APT H-bonding electrostatic interactions. Antibacterial evaluation results indicate MAG/C–ZnO/APT with doses 2.5% 0.25%, respectively, exhibited synergistically enhanced inhibitory activities against Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, methicillin-resistant aureus extended-spectrum ?-lactamases minimum concentrations 1, 0.1, 0.25, 5, 2.5 mg/mL, which are better than those ZnO/APT, C–ZnO/APT MAG. Moreover, had low cytotoxicity human normal cell line L-O2. Therefore, study provided more effective strategy extend spectrum strengthen effects antibiotic-free materials address increasingly serious situations microbial infection.